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Office Location:
Salk Pavilion 508

Mailing address:
335 Sutherland Drive
Pittsburgh, PA 15261

Email: gii5@pitt.edu

Phone: 412-648-8535

Other Affiliations:
Center for Craniofacial Regeneration; Department of Oral and Craniofacial Sciences; Department of Medicine/Division of Hematology-Oncology; Hillman Cancer Center; McGowan Institute for Regenerative Medicine

MEMBERS

Giuseppe Intini, DDS, PhD

PCIBMR Co-Director
PCIBMR Advanced Bioimaging Core Co-Director

Associate Professor, Department of Periodontics and Preventive Dentistry
University of Pittsburgh School of Dental Medicine

Research Interests

The research in the INTINILAB focuses on skeletal stem cells and of bone cancer stem cells. Genetic strategies and in vivo imaging are utilized to describe the location and function of these cells and the molecular mechanisms that control their “stemness” in health and disease.

Technique Expertise or Resources to share

The laboratory has identified the calvarial suture as the niche of osteoprogenitor cells expressing Prx1, a transcription factor highly expressed during embryonic development. Using various transgenic mouse models, current research aims at taking advantage of the existence of these cells to develop novel strategies to foster craniofacial tissue regeneration. Additional projects focus on strategies to utilize Prx1 expressing cells for regeneration of other skeletal regions. A federally funded project (NIH/NCI) supported the generation of an osteosarcoma multifluorescent mouse model which allows for studying the role of cancer stem cells in the development, maintenance, and metastasis of osteosarcoma.

In vivo mouse imaging (intravital microscopy), focusing on the development of assays for in vivo tracing of fluorescent stem cells and their progeny during tissue regeneration or during cancer development and metastasis. 10X Genomics single cell RNAseq and bioinformatic analyses. Mouse models: Prx1-Cre (Prrx1-Cre); Prx1-creER-EGFP; Osx-mCherry; Col1-CFP; β-catenin floxed; p53 floxed; Rb1 floxed; ROSA-DTA floxed; ROSA-td-TOMATO floxed. Cell lines: pre-osteoblast cell lines; osteosarcoma cell lines.

Keywords

Osteoblasts, skeletal stem cells, mesenchymal stem cells, stem cell niche, bone regeneration, periodontal regeneration, tissue regeneration, Prx1, Prrx1, osteosarcoma, osteosarcoma metastasis, intravital microscopy, in vivo microscopy, fluorescent microscopy.

Laboratory

Lab website: https://www.dental.pitt.edu/person/giuseppe-intini

 

Lab Members

Xuehui Geng, MD, MS Research Scientist xuehui@pitt.edu
Jue Hou, PhD Postdoctoral fellow JHOU1@mgh.harvard.edu
Luigi Mancinelli, PhD Postdoctoral fellow lum39@pitt.edu
Roberta Di Carlo, PhD Postdoctoral fellow
Taiana Campos Leite, DDS Post-graduate student tal117@pitt.edu
Mazat Alafandi, BS Graduate student maa386@pitt.edu